Liens utiles

Générale

• Howard M. Shapiro: Practical Flow Cytometry
• Michael G Ormerod: Flow Cytometry - A Basic Introduction
• Tim Bushnell: Modern Flow Cytometry
• Guidelines for the use of flow cytometry and cell sorting in immunological studies

Instrumentation

• Photodetectors in Flow Cytometry
• Gains versus Voltage Explained
• CytoFlex Manual

Tutorial

• Introduction to Flow Cytometry
• Analyzing Flow Cytometry Data
• Peripheral Blood Mononuclear Cell (PBMC) Processing

Panel builder

• Fluorofinder:Antibodies & Fluorochromes selector une excellente ressource
• Antibodies selector une ressource par excellence
• Fluorophore Equivalency

Overcoloring

• Sentinel Panel Design of 16-Color, 28-Markers for Immunophenotyping Peripheral Human Whole Blood
• Extending instrument capability: Sentinel Marker Approach
• Human and Mouse CD Marker Handbook

Spectrum viewers

• BD Biosciences
• Invitrogen
• BioLegend Spectra Analyzer

Fluorescent Proteins

• Fluorescent protein properties
• Photoswitchable Fluorescent Protein Properties
• Fluorescent Proteins Table

Fc (CD16/32) blocking

• Fc block treatment, dead cells exclusion, and cell aggregates discrimination concur to prevent phenotypical artifacts in the analysis of subpopulations of tumor‐infiltrating CD11b+myelomonocytic cells
• Three blocks are not enough—Blocking of the murine IgG receptor FcγRIV is crucial for proper characterization of cells by FACS analysis
• Elimination of erroneous results in flow cytometry caused by antibody binding to Fc receptors on human monocytes and macrophages

Compensaton

• Antibody Capture Beads
• Affinity of proteins A and G
• Compensation of spectral overlap